12.5.3. Study design

The RFID technology allows detailed analysis and comparison of various foraging flight parameters (see section 11) between different groups of bees. It is a very sensitive method for registering even subtle effects of stressors on forager behaviour, vitality and viability. Thus, in designing experimental groups, great care is required to randomize any treatment influence in the preparatory steps. In addition, handling of the bees should be performed very carefully to avoid unnecessary stress during treatment administration, which might cover treatment effects.