2.4. Harnessing individual bees
For a number of behavioural assays,
honey bees need to be harnessed individually. Metal or plastic holders (Fig. 2A)
have proved very useful for mounting bees, and they can be produced easily.
Bees are placed in a holder while being narcotized. A strip of textile tape
between head and thorax fixes the bee. A second strip of tape fixed over the
abdomen prevents the experimenter from getting stung. If bees are supposed to
walk freely after their confinement to the holder, a plaster should be used
instead of textile tape to save the wings and legs from sticking to the tape.
For more details on immobilizing bees, refer to the section ‘Standard methods
for immobilising, killing and storing adult Apis
mellifera in the laboratory’ in the BEEBOOK
paper on miscellaneous methods (Human et