To summarize, the most important features of a volatile collection and analysis design are to:
- Isolate the sample of interest as much as possible without disturbing it.
- Collect emitted volatiles with a technique that is as sensitive and efficient as possible while limiting the introduction of contaminants.
- Collect background samples to help identify outside contaminants in the samples.
- Use appropriate analytical techniques to detect and evaluate the biologically-important compounds of samples.
- Confirm compound identities and quantities with appropriate chemical standards and techniques.
- Test the biological activities of samples with
bioassays on isolated native material.
Then confirm the activities of suspected compounds with bioassays on
synthetic chemical standards.