4.7.2. Methods for pollen collection
These methods are mostly used for studies on pesticide
residues (Dively and Kamel, 2012, see also the BEEBOOK paper on toxicology, Medrzycki et al.,
2013) and nutritional content of pollen (e.g., Human and Nicolson, 2006; see
references therein). Fresh pollen can be collected directly from flowers where
the bees are foraging. There are three basic examples of fresh pollen
collection; using bags over the flowers (section 18.104.22.168.1.), by physically
shaking the flowers over plastic trays (section 22.214.171.124.2.) or by gently
brushing off the pollen from the male anthers with a paint brush (section 126.96.36.199.3.).
Whenever fresh pollen is to be collected, flower buds that are open and ready to start shedding pollen, need to be
covered with fine gauze or pollination bags the day before collection in order
to prevent insect visitation and thus possible contamination. Bee collected
pollen can be collected with pollen traps at the hive entrance or manually from
the combs in which it has been stored (as bee bread).