11.6. Methylation dynamics and expression of individual genes

Targeted analyses of selected genes can be conducted using 454 sequencing of amplified gene fragments from bisulfite-converted DNAs. For a small-scale testing the amplicons can be cloned into a plasmid, cloned and sequenced using via Sanger dideoxy sequencing (Foret et al., 2009). Both approaches can be used to validate genome-wide methylation data, but 454 sequencing allows for a much higher coverage, as shown in section 8.5.

11.6.3. Bisulfite PCR