11.7. RNA extraction
- RNA for analysis can be extracted using the TRIzol®/QIAGEN RNeasy combination method followed by the QIAGEN Mini or Rneasy Minelute Cleanup kit, or by the detailed protocol in the RNA methods section 4.3. above.
- RNA concentrations are then evaluated via Nanodrop (section 3.2.1) analyses and integrity assessed by gel electrophoresis (see section 4.4.).