An excellent, cheap alternative to the beadmills, especially for large volumes is homogenisation with a blender.
- Pros: Large volume; rapid; uniform.
- Cons: Cross-contamination risk due to re-use of
blender; incompatible with organic solvents; corrosion of blender due to salts.
- Add between 30-200 frozen bees to blender.
- Add 500 μl ice-cold buffer per bee.
- Homogenise by gradually raising the blender settings, for about 5 minutes total homogenization.