You will need individual disposable micro pestles that fit microcentrifuge tubes. These can be bought or made by heating a 1000 μl blue tip in a flame and moulding it into a disposable pestle in a microcentrifuge tube while it cools.
- Pros: Single bees; cheap; low maintenance.
- Cons: Time consuming;
lack of uniformity.
- Grind a frozen bee tissue or larval sample with the micropestle in a microcentrifuge tube.
- Discard pestle.
- Add 500 μl buffer per bee.
- Mix with a vortex.