Fig. 4. Rocket immunoeletrophoresis for quantification of haemolymph vitellogenin in 1 to 6 day-old Apis mellifera workers reared on different diets. Haemolymph was from bees fed a 0 % pollen diet (wells 1-4), a 15 % pollen diet (wells 5-7), a 50 % pollen diet (wells 8-10), a pollen-free sugar diet supplemented with soybean and yeast (wells 11-13), and naturally fed workers (wells 14-16). Reproduced from Bitondi and Simões (1996). Copyright Journal of Apicultural Research.