220.127.116.11.2.6. Dust application
1) 0.01 g of total dust (the AI-containing dust plus the talc powder) per cage should be distributed on the leaves (Table 4). This quantity was considered adequate for a homogeneous distribution on the surface of approximately 50-70 cm2. For bigger cages, a proportionally higher amount should be used.
2) A small sieve obtained from a modified Eppendorf
tube can be used as shown in Fig. 1.
Fig. 1A. Small sieve obtained from a modified Eppendorf tube (the bottom is removed from the tube and replaced with screen mesh); B. dust application in the apple leaves; C. leaves placed in the bottom of the hoarding cage.