Investigating the potential of SHB to vector honey bee viruses

To investigate the potential of SHB as a vector of honey bee viruses, SHB can be fed with virus-infected workers or brood or with virus-contaminated wax or pollen. Eyer et al. (2009a,b) found no virus infections through feeding of contaminated pollen, but SHBs became infected through feeding on infected worker or brood and on contaminated wax:

  1. Maintain laboratory-reared adult SHB (see section 3.1.1. and Fig. 8 & 12) and provide virus contaminated food or other material.
  2. Collect adult SHB after 6 days and store adequately until virus analysis (see respective BEEBOOK paper by de Miranda et al., 2013).