2.4.1. DNA extraction
DNA is sourced from the tissue of Varroa spp. mites that have been collected and preserved in 70 % ethyl alcohol or RNAlater (as described in section 18.104.22.168. ‘Storage medium and conditions’). Any tissues can be used, but if the tissue is dissected from a single appendage (such as a leg), the rest of the mite can be used for other purposes. See the section on ‘CTAB genomic DNA extraction from adult bees’ of the BEEBOOK paper on molecular methods (Evans et al., 2013) for extraction methods.