In the field

1. Treat brood cells in colonies with the stimulus under testing or treat mites.

2. Open cells a minimum of 70 h after mite introduction.

3. Investigate the presence of eggs in the cells.

Opening the cells later (e.g. between 7 and 10 days) will facilitate detection of eggs and offspring, but increase the chances of removal by hygienic workers.


Pros: less time consuming compared to laboratory bioassay (see section Many brood cells can be treated under natural conditions.

Cons: Stimuli cannot be tested independently from other factors (larvae, nurse bee activity).