3.1. Natural rearing method
- Create a bee-free hive with frames of pulled, dark comb (dark comb is comb in which brood has been reared) containing honey and pollen.
- Introduce three, late instar larval wax moths per frame to ensure wax moth presence (Hood et al., 2004).
- The hive and combs should be covered and under some type of shelter to protect it from rain. Darkness, warmth, and lack of ventilation promote colonization.
- Unattended (bee-free) hives will be highly attractive to adult wax moths if they are present in the area (Hood et al., 2004)
- Provide additional used honeycomb containing honey and pollen as diet for rearing program as the food supply in the box is exhausted.
- Moth eggs, larvae, pupae and adults can be collected from the hive with an aspirator, forceps, or a small, soft paintbrush. The latter should be used for the immature wax moth stages since they can be damaged easily.