6.2.3. Removing haemocytes from haemolymph
Per Fröbius et al. (2001):
- Collect haemolymph from wax moth larvae per Section 6.2.1.
- Transfer the haemolymph to chilled tubes. The tubes should contain a few crystals of phenylthiourea to prevent melanization (Vilcinskas et al., 1997, Wedde, et al., 1998).
- Centrifuge the haemolymph twice at 100 g for 10 min to remove the haemocytes.
- Store the supernatants at -20°C until needed.